Email this article Study of level of malondialdehyde and total antioxidant capacity (TAC) in human lens epithelial cells of diabetic and senile cataract patients
Abstract
Background: Oxidative stress is a condition where the balance between the production and elimination of reactive oxygen species (ROS) is disturbed. ROS can cause damage to various biomolecules, including DNA. DNA damage can impair the function and survival of cells, and may contribute to the development of cataracts. Several experimental studies have shown that oxidative stress is involved in cataract formation by inducing DNA damage in lens cells.. ROS are created in diabetic tissues by glucose autoxidation as well as non-enzymatic protein glycation. ROS is thought to play a significant role in the development of microvascular problems in diabetic individuals
Objectives: To measure the levels of total antioxidant capacity and Malondialdehyde (MDA) in diabetic and nondiabetic senile cataract patients and comparison between them.
Patients and methods: Thirty Senile cataract cases and 30 diabetic cataract cases aged 50-80 years who were brought to the ophthalmology ward of Acharya Vinoba Bhave Rural Hospital for cataract surgery were used for TAC assay and malondialdehyde determination in lens epithelial cells of diabetic and senile cataract.
Results: TAC was decreased in human lens epithelial cells of diabetic and senile cataract group. TAC is more reduced in diabetic than senile cataract group, it is not statistically significant. higher levels of MDA in human lens epithelial of Diabetic cataract patients as compared to senile cataract patients.
Conclusion: The conclusion in the present study diabetes may cause ocular complications by affecting the lens of the eye. This is because diabetic patients have lower levels of TAC and higher levels of Malondialdehyde in their lens than senile cataract patients. These factors may contribute to pathogenesis of diabetic cataract.
Received: 23 June 2023
Accepted: 09 August 2023
Published: 25 August 2023
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Nizami AA, Gulani AC. Cataract. 2023.
Yildirim Z, Yildirim F, Ucgun NI, Kilic N. The evaluation of the oxidative stress parameters in nondiabetic and diabetic senile cataract patients. Biol Trace Elem Res. 2009;128(2).
Niedernhofer LJ, Daniels JS, Rouzer CA, Greene RE, Marnett LJ. Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells. Journal of Biological Chemistry. 2003;278(33).
Katta A V., Katkam R V., Geetha H. Lipid peroxidation and the total antioxidant status in the pathogenesis of age related and diabetic cataracts: A study on the lens and blood. Journal of Clinical and Diagnostic Research. 2013;7(6).
Javadi MA, Zarei-Ghanavati S. Cataracts in diabetic patients: A review article. Vol. 3, Journal of Ophthalmic and Vision Research. 2008.
Aksoy H, Keles S, Koçer I, Akçay F. Diabetic cataract and the total antioxidant status in aqueous humor. Clin Chem Lab Med. 2001;39(2).
Nita M, Grzybowski A. The Role of the Reactive Oxygen Species and Oxidative Stress in the Pathomechanism of the Age-Related Ocular Diseases and Other Pathologies of the Anterior and Posterior Eye Segments in Adults. Vol. 2016, Oxidative Medicine and Cellular Longevity. 2016.
Kiziltoprak H, Tekin K, Inanc M, Goker YS. Cataract in diabetes mellitus. World J Diabetes. 2019;10(3).
Wormstone IM, Wormstone YM, Smith AJO, Eldred JA. Posterior capsule opacification: What’s in the bag? Vol. 82, Progress in Retinal and Eye Research. 2021.
Nespolo M, Halliwell B, Gutteridge C, John MC, Gutteridge JM. Free Radicals in Biology and Medicine. Fifth Edition. Acta Cryst. 2017;73.
Giacco F, Brownlee M. Oxidative stress and diabetic complications. Vol. 107, Circulation Research. 2010.
Chang D, Zhang X, Rong S, Sha Q, Liu P, Han T, et al. Serum antioxidative enzymes levels and oxidative stress products in age-related cataract patients. Oxid Med Cell Longev. 2013;
Kaur J, Kukreja S, Kaur A, Malhotra N, Kaur R. The oxidative stress in cataract patients. Journal of Clinical and Diagnostic Research. 2012;6(10).
Berthoud VM, Beyer EC. Oxidative stress, lens gap junctions, and cataracts. Vol. 11, Antioxidants and Redox Signaling. 2009.
Hilliard A, Mendonca P, Russell TD, Soliman KFA. The protective effects of flavonoids in cataract formation through the activation of nrf2 and the inhibition of mmp-9. Vol. 12, Nutrients. 2020.
Balasubramanian D, Bansal AK, Basti S, Bhatt KS, Murthy JS, Rao CM. The biology of cataract. The Hyderabad Cataract Research Group. Vol. 41, Indian journal of ophthalmology. 1993.
Murthy GVS, Gupta S, John N, Vashist P. Current status of cataract blindness and Vision 2020: The right to sight initiative in India. Indian J Ophthalmol. 2008;56(6).
Ghannam JY, Al Khazari KA. StatPearls Publishing. 2022. Neuroanatomy, Cranial Meninges.
DOI: http://dx.doi.org/10.21622/AMPDR.2023.03.2.012
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Copyright (c) 2023 Kranti Santosh Sorte Gawali, Avinash Namdeo Jadhao
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Advances in Medical, Pharmaceutical and Dental Research
E-ISSN: 2812-4898
P-ISSN: 2812-488X
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Arab Academy for Science, Technology and Maritime Transport (AASTMT)
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